Table 3.
Mass Spectrometry Proteomics Analysis.
| Protein Detected | UniProtKB # | Porcine Scaffold Samples | Human DBM Samples | Protein Function |
|---|---|---|---|---|
| Chondroadherin | F1RT93 O15335 |
3/3 | 3/3 | Promotes the attachment of chondrocytes, fibroblasts, and osteoblasts. JAK-STAT cascade signaling |
| Collagen alpha-1(I) chain | P02452 | 3/3 | 3/3 | Protease binding, metal ion binding, bone trabeculae formation, enchondral ossification, cell response to TGF β, collagen fibril organization, cell response to mechanical stimuli, and osteoblast differentiation |
| Collagen alpha-2(I) chain | A0A1S7J1Y9 P08123 |
3/3 | 3/3 | Extracellular matrix structural constituent, SMAD signaling, collagen fibril organization, cytokine signaling, and TGF β receptor signaling |
| Pigment epithelium-derived factor |
Q0PM28 P36955 |
3/3 | 3/3 | Neurotrophic protein, inhibitor of angiogenesis, cell proliferation |
| Serum albumin |
P08835 P02768 |
3/3 | 3/3 | Main plasma protein |
| Alpha-2-HS-glycoprotein |
P29700 P02765 |
3/3 | 2/3 | Endopeptidase inhibitor; negative regulation of biomineral tissue development; negative regulation of bone mineralization, positive regulation of phagocytosis |
| Lumican; fibromodulin |
Q9TTB4 P51884 |
3/3 | 2/3 | Primary role in collagen fibrillogenesis, collagen binding, response to growth factor |
| Biglycan |
Q9GKQ6 P21810 |
2/3 | 3/3 | Involved in collagen fiber assembly, protein kinase inhibitor, negative regulation of JAK-STAT cascade, ECM structure, blood vessel remodeling, and cytokine signaling |
| Annexin A5 | P08758 | 3/3 | 0/3 | Blood coagulation |
| Hemoglobin subunit beta |
P02067 P68871 |
2/3 | 0/3 | Highly abundant blood protein, oxygen transport |
| Alpha-1-antiproteinase |
P50447 P01009 |
2/3 | 0/3 | Serine protease inhibitor; found in high levels in blood |
| Vitronectin |
P48819 P04004 |
0/3 | 3/3 | Cell adhesion and spreading factor in serum and tissues, cell proliferation, wound healing, and cell migration |
| Prothrombin | F1SIB1 P00734 |
0/3 | 2/3 | Blood coagulation, converts fibrinogen to fibrin, activates coagulation factors |
Mascot protein scores >100 (www.matrixscience.com) are listed with respective species-specific UniProtKB accession numbers. Three separate porcine scaffold and three human DBM samples were separately analyzed and the number of these samples recording hits for the listed proteins is reported. Proteins observed in only one sample or those which had protein scores <100 were excluded from analysis. Functional information of reported proteins was derived from the UniProtKB database [40].