miRNA expression profiling using TaqMan miRNA Array (Thermo Fisher Science, Waltham, MA, USA). Exosome fraction taken from plasma samples of 10 patients who underwent allogeneic HSCT (5 late-onset acute GVHD and 5 non-GVHD), with 8 normal controls being included. A differential expression pattern was found between late-onset acute GVHD patients and non-GVHD patients. Using SDS (version 2.4) and DataAssist software (version 3.01, Thermo Fisher, version, Waltham, MA, USA), we calculated the expression of miRNAs based on their Ct value normalized by the Ct value of ath-miR-159. Data were analyzed with GeneSpring software.