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. 2018 Sep 11;19(9):2697. doi: 10.3390/ijms19092697

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of Tacrolimus (FK506) on multidrug resistance-associated protein 1 (MRP1) levels in U87MG and C6 cells. Cells were treated with FK506 (7, 15, and 30 ng/mL) for 24 h. MRP1 mRNA levels were measured by RT-qPCR in U87MG cells (A) and C6 cells (B). Graphs represent the values normalized by actin beta (ACTB). MRP1-positive cells were quantified by flow cytometry in U87MG cells (C) and C6 cells (D). White bars represent non-glioblastoma stem-like cells (GSCs) and black bars represent glioblastoma stem-like cells (GSCs).