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. 2018 Jun 26;7(3):37. doi: 10.3390/biology7030037

Figure 4.

Figure 4

(a) Characterization of OXPHOS proteins expressed in HepG2 cells during high glucose with or without M. oleifera extract. OXPHOS cocktail specificity demonstrated by Western blot of MTC01 subunit of CIV, SDHB subunit of CII, UQCRC2 subunit of CIII, NDUFB8 subunit of CI, and ATP5A subunit of CV. BN-PAGE. Activity of CI (b) and CIV (c) in isolated mitochondria from HepG2 cells under normal (C), glucose 25 mM (G), and 500 µg/mL M. oleifera extract (GM) conditions. * significant difference against control p ≤ 0.05. + significant difference against G cells p ≤ 0.05. Data normalized to control group using β-actin.