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Effects of intracellular delivery of CA-ESR1 siRNA complexes on expression and activation of estrogen receptor α in MCF-7, MDA-MB-231 and 4T1 cell lines. Cells were incubated with CA-ESR1 complexes for 48 h prior to cell lysis for protein extraction and Western blot analysis. Proteins were loaded on SDA-PAGE and transferred onto nitrocellulose membrane for detection of phosphorylated estrogen receptor (ER) α, total ER α and GAPDH (housekeeping protein) expression.
