Figure 5.

Cell viability and cytotoxicity assessments of 4T1 cells treated with various CA-siRNA complexes involving ESR1, ERBB2, IGF1R, EGFR and BCL2 siRNAs (1 nM) individually or in combination, for a period of 48 h. Preparation of the CA-siRNA(s) complexes involved introduction of ESR1, ERBB2, IGF1R, EGFR and BCL2 siRNAs individually or in combination with 3.5 mM of CaCl₂ to 1 mL of bicarbonate-buffered DMEM (pH 7.4). The mixture was allowed incubation at 37 °C for 30 min before the addition of 10% FBS. The cells were incubated for the next 48 h with the prepared complexes. MTT assay was performed and absorbance reading was taken at 595 nm with 630 nm as references wavelength. Data was presented as mean ± SD of triplicates.