Figure 3.

Effects of chronic insulin and LCn-3PUFA on insulin signaling in mature adipocytes. 3T3-L1 preadipocytes were differentiated for eight days before a 48 hour-treatment with 10 µM docosapentaenoic acid (DPA) or 50 µM eicosapentaenoic acid (EPA). Insulin resistance was induced by adding insulin (10 µM) during the last 16 h of treatment (chronic insulin, CI). Cells were then starved for 6 h. Before harvesting, cells were stimulated with insulin (100 nM) to evaluate insulin sensitivity by the quantification of Akt protein phosphorylation. Data are mean ± SEM (n = 10 in CI and control (CTRL) groups and n = 20 in LCn-3PUFA groups obtained after four experiments.) * p < 0.05 vs. CI and *** p < 0.001 vs. CI were obtained by ANOVA followed by the Dunnett post-hoc test.