Figure 5.

Effects of chronic insulin and LCn-3PUFA on adipokine secretion by mature adipocytes. 3T3-L1 preadipocytes were differentiated for eight days before a 48-hour treatment with 10 µM docosapentaenoic acid (DPA) or 50 µM eicosapentaenoic acid (EPA). Insulin resistance was induced by adding insulin (10 µM) during the last 16 h of treatment (chronic insulin, CI). Cells were then starved for 6 h and conditioned media were collected for quantification of IL-6 (A), MCP-1 (B), CCL5 (C), and adiponectin (D) concentrations. Data represent the mean ± SEM (n = 8–12 obtained after three experiments). * p < 0.05; ** p < 0.01; and *** p < 0.001 vs. CI obtained by ANOVA followed by the Dunnet post-hoc test. CCL-5: C-C motif chemokine ligand 5; IL-6: interleukin-6; MCP-1: monocyte chemoattractant protein-1.