Figure 6.

Effects of adipocyte condition media on Akt activation in skeletal muscle cells. Adipocyte media were collected after 48 h of treatment with, or without, 16-hour chronic insulin (CI), docosapentaenoic acid (DPA), oreicosapentaenoic acid (EPA), and after six hours of starvation. C2C12 muscle cells were treated for 16 h with conditioned media (CM). Before harvesting, cells were stimulated with insulin (100 nM) to evaluate insulin sensitivity by the quantification of Akt protein phosphorylation. Data are represented as the mean ± SEM (n = 10 in CI and control (CTRL) groups, and n = 20 in LCn-3PUFA groups obtained after four experiments. ** p < 0.01 vs. CI obtained by ANOVA followed by the Dunnet post-hoc test.