Skip to main content
. 2018 Aug 22;10(9):1141. doi: 10.3390/nu10091141

Figure 6.

Figure 6

Downregulation of EGFR and p1068EGFR expression by EGCG and IIF treatments in BE(2)-C neuroblastoma cells. Cells were treated with 20 μg/mL EGCG and 10 μM IIF, individually and in combination for 24 h. (A) Proteins (50 μg) from total cell lysate were subjected to SDS–PAGE and Western blot analysis of EGFR and p1068EGFR expression after 24 h treatments. Actin was used as a loading control. RT-PCR analysis of EGFR (B) and NDRG1 (C) in control and treated cells. β-actin was used as a control. The values were normalized to the untreated controls. The results are expressed as the average ± SE of three independent experiments. * p < 0.05; ** p < 0.01. n.s.: not significant. WB: western blot; RT-PCR: reverse transcriptase-polymerase chain reaction.