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. 2018 Aug 22;10(9):1141. doi: 10.3390/nu10091141

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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EGCG and IIF treatments limited sphere formation and N-MYC expression in BE(2)-C neuroblastoma cells. (A) Neural cancer stem cells (NCSC). Parental adherent BE(2)-cells were grown in non-adherent serum-free conditions to develop spheres for 72 h (CTR) and treated with 20 μg/mL EGCG and 10 μM IIF, individually and in combination for 72 h. Viability was evaluated by Trypan blue assay. (B) N-MYC mRNA expression was evaluated by qPCR in control (CTR) and treated cells. GAPDH was used as a loading control. The results are expressed as the average ± SE of three independent experiments. * p < 0.05. n.s.: not significant.