Table 1.
Nucleotide sequence of primers used for the quantitative polymerase chain reaction (qPCR) assay.
Primer Name | Nucleotide Sequences (5′-3′) | Product Size | Position | Reference |
---|---|---|---|---|
F-Omt-1 | GGCCGCCGCTTTGATCTAGG | 123 | a 1485 | [42] |
R-Omt-1 | ACCACGACCGCCGCC | 1593 | ||
F-foxA | ACCGCAACCCTCTTCACATT | 73 | b 1196 | This study |
R-foxA | AGACCGTGCAGGATAGGAGT | 1268 | ||
F-β-tub | TCTTCATGGTTGGCTTCGCT | 98 | c 964s | This study |
R-β-tub | CTTGGGGTCGAACATCTGCT | 1042 |
a Positions are in accordance with the published sequence of the aflP (omt-1) gene of A. flavus (GenBank accession no. L25835.1). b Positions are in accordance with the published sequence of the foxA gene of A. flavus (GenBank accession no. XM_002377580). c Positions are in accordance with the published sequences of the β-tubulin gene of A. parasiticus (GenBank accession no. FR775333.1).