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. 2018 Sep 7;19(9):2662. doi: 10.3390/ijms19092662

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of mucins on zinc availability for 4-(2-pyridylazo)resorcinol (PAR). Effect of mucins on zinc availability for PAR is shown as free zinc relative to the initially added zinc concentration. Different concentrations of zinc-depleted porcine mucin were incubated with 8 μM zinc and free zinc was analyzed using the colorimetric zinc chelator PAR. Data are presented as means + standard deviation (SD) of at least three independent experiments. Significant differences to the control are indicated (*** p < 0.001; one-way analysis of variance (ANOVA) with Dunnett’s multiple comparison test).