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. 2018 Sep 7;19(9):2662. doi: 10.3390/ijms19092662

Figure 2.

Figure 2

Zinc binding properties of gastrointestinal mucins. Different zinc concentrations were added to 25 mg/mL zinc-depleted porcine mucins and dialyzed against (Tris)-buffered saline (TBS) for 12 h. Subsequently the amount of zinc retained by binding to mucins was measured using flame atomic absorption spectrometry (FAAS) (A). Moreover, the zinc content of mucins after zinc loading is shown relative to the glycoprotein content measured by quantitative periodic acid Schiff (PAS)-assay (B), and relative to protein content of mucins measured by bicinchoninic acid (BCA)-assay (C). Data are shown as means ± SD of at least three independent experiments. Significant differences to the control are indicated (** p < 0.01; *** p < 0.001; one-way ANOVA with Dunnett’s multiple comparison test).