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. 2018 Sep 7;19(9):2662. doi: 10.3390/ijms19092662

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Zinc binding affinity of gastrointestinal mucins. Shown are zinc binding affinities of two different zinc-depleted mucins analyzed with the chromophore Zincon. For this, 1 mg/mL porcine mucins (A) and mucins harvested from HT-29-MTX (B) were used. Data were analyzed with GraphPad Prism software version 5.01 (GraphPad Software Inc., San Diego, CA, USA) and a non-linear regression assuming a one site-specific binding with Hill slope as a function of the zinc concentration was applied to calculate the dissociation constants of the mucin-zinc-complex as indicated. Data are presented as means ± SD of three independent experiments.