Skip to main content
. 2018 Sep 7;19(9):2662. doi: 10.3390/ijms19092662

Figure 8.

Figure 8

Comparison of zinc resorption in Caco-2 monocultures and Caco-2/HT-29-MTX co-cultures. Zinc transport studies were conducted using the enterocytes Caco-2 (A,C,E) and a co-culture of Caco-2 with the mucus-producing goblet cell line HT-29-MTX (B,D,F). Shown are the decrease of apical zinc (apical zinc uptake) (A,B) and fractional zinc resorption after 4 h incubation relative to the initially added amount of zinc (C,D). Moreover, cellular zinc uptake is shown relative to cellular protein content (E,F). Data are shown as means + SD of three independent experiments and means significantly different from the untreated controls are indicated (* p < 0.05; ** p < 0.01; *** p < 0.001; one-way ANOVA with Dunnett’s multiple comparison test). According to a two-way ANOVA with the Bonferroni post hoc test comparing the results within one added zinc concentration of the mono- and co-cultures there are significant differences regarding the fractional zinc resorption (25 µM: p < 0.001; 50 µM: p < 0.05), and zinc uptake (50 µM: p < 0.05).