Table 5.
Application of primers and sequences. ORF: open reading frames.
| Use | Primer Name | Forward Primer (5′–3′) | Reverse Primer (5′–3′) |
|---|---|---|---|
| Complete | MdSAP7 | ATGAAAAAAATGGCACAGAGAA | TCAAACCCGGACGATCTTTGCGG |
| ORF | MdSAP8 | ATGGAGCACAATGAGACAGGAT | TCAGATTTTATCCAGCTTTTCT |
| amplification | MdSAP10 | ATGGAGCACGAGGAGACTGGATG | TTAGATTTTATCGAGCTTCTCA |
| MdSAP12 | ATGGCGGAAGAGCACAGATGCG | TCAAATCTTCTCGAGCTTCTCG | |
| MdSAP14 | ATGAAAAAAATGGCACAGAGAA | TCAGAGCCGGACGATCTTCGCA | |
| MdSAP15 | ATGGAGCACAATGAGACAGGATG | TCAGATTTTATCCAGCTTGTCTG | |
| MdSAP16 | ATGGAATCTCATGATGAAACTG | CTAGATTTTGTCAAGTTTGTCTG | |
| MdSAP19 | ATGGCGGAAGAGCATCGTTGCCA | TCAAATCTTATGCAGCTTCTCCG | |
| MdSAP21 | ATGATGGGAGGAACAGAAGCTT | TCAATACGCTCGAACAGATGGCC | |
| MdSAP23 | ATGGAGCACGAGGAGACTGGATG | TTAGATTTTACCAAGCTTGTCAG | |
| MdSAP25 | ATGGGAACTCCGGAATTCCCAGA | CTATGCTCTTGAAGTACCGCCGT | |
| MdSAP28 | ATGACGGGAGGAACAGAAGCTTT | TCAATAAGCTCGAACAGAAGGC | |
| MdSAP29 | ATGGCGGAAGAGCACAGATGCGA | TCACGGATTTGTACGTTGGCAA | |
| Promoter | MdSAP7 | CAGATTTTGTTCAAATGTAGG | TGGGCGATGGAGGAGACAGAAAT |
| amplification | MdSAP8 | TGTTTCAATTGCGTTCTTGAGG | CATTGTAATTCGCTTAAGTTCT |
| MdSAP10 | ACCTTTTCCAAAACCGTTATTAG | TGCGAAAACCAACAATTAATGG | |
| MdSAP12 | |||
| MdSAP14 | GTAAAGAGGTTAGTGGCCCTGAA | CAAATTCTGATCGATCGATCGAT | |
| MdSAP15 | ATGCGCTTTACTGTTTTTTCAGT | CATTGTAATTCGCTAAGTCCTT | |
| MdSAP16 | CACGAGGAGAGCACTAAAATGGA | CACCAAGAAAACCTCGCCGTTT | |
| MdSAP19 | ACCTTTCTTTTGAGAAGTTTGT | TGCAATTCCAAAACAAATTATTC | |
| MdSAP21 | ATGGATTCTAGTTTGATTTGGGC | GATTTTTCAGTTTGTTAAATTTT | |
| MdSAP23 | ATATTTCCATCACATTGAATAA | CTACTCAGCTTACCTGCAAAGAG | |
| MdSAP25 | GCAGGTAGAGTTTCAAAGTACG | AAATTTTGTATGTACAACACTA | |
| MdSAP28 | ACAGGTCACCGTGGTGACTCCGG | GTCGGTCGGTCGGTCTGGGGTTG | |
| MdSAP29 | GTGCTTTTTGTTGGAACACAAAG | CGATCGAGAGGACAAAAATATTA | |
| qRT-PCR | MdSAP7 | TCGTCCGGGTTTGATGATTT | TCCCCGGTCTCTGAATTTCG |
| MdSAP8 | GGGAAGCGGATAGGAACCAT | CTTGGGAGCTTCAGGAGGAG | |
| MdSAP10 | GATTATCGCACTGCTGGACG | AGTGCTAAGATACCGCTGCA | |
| MdSAP12 | GTTGGTCATAGCCGAGAAGC | ATCAGCTTAATTCCCACGCG | |
| MdSAP14 | GCTCTGACCGGTTTGACAAT | TTGCTGATGATCTCCGGGAG | |
| MdSAP15 | ATGATTACCGGACTGCTGCT | CCACATGGGTAGAAATGAGAGC | |
| MdSAP16 | GCCAATCCTATCGTGAAGGC | GAGACCTATGCAGACAAGAAGC | |
| MdSAP19 | CGATTTCAGAGGGATGGGGA | CAACCATCCCCTACCCCAAT | |
| MdSAP21 | AGGGAAAGAATGCGGGAAGA | CGAAGAAACATGAAACTGCGG | |
| MdSAP23 | GCCAACCCTGTCGTAAAAGC | TGCTAAGATACCGCTGCAGA | |
| MdSAP25 | AATCCAATCCAAGCCTCGGA | TCCCATCCGAATTTTGCACG | |
| MdSAP28 | TGCTTTGAGGGAAGGGAAAGA | ACATCGAATTGTGGAAGCAGA | |
| MdSAP29 | TTCCTCCTCGCACAGATCAG | TCCGCCATGTCTACAGTCAA | |
| MdMDH | CGTGATTGGGTACTTGGAAC | TGGCAAGTGACTGGGAATGA | |
| pRI-101AN | MdSAP15 | TTGATACATATGCCCGTCGACATGGAGCACAAT | AGAGTTGTTGATTCAGGATCCTCAGATTTTATC |
| 35S | CGCACAATCCCACTATCCTT | ||
| qRT-MdSAP15 | AGTCGTTGCAGCATCCATTG | GGAAGCCTGTGTTGAGATAAGC | |
| AtActin2 | GTGAAGGCTGGATTTGCAGGA | AACCTCCGATCCAGACACTGT |