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. 2018 Aug 1;8(3):67. doi: 10.3390/biom8030067

Figure 6.

Figure 6

Increased levels of α-synuclein protein. SH-SY5Y (human neuroblastoma) cells were treated for 24 h with serum free medium (control) conditioned medium from control (CM) and iron-fed microglia (Iron-fed CM). The level of α-syn expressed by SH-SY5Y cells was assessed by western blot using a specific antibody. Protein loading levels were assessed using western blot for tubulin. The effect of conditioned medium from either (A) C8B4 or (B) Primary microglia was assessed and densitometric analysis carried out (C). Compared to SH-SY5Y cells grown in serum free medium (control) conditioned medium from control microglia (CM) had no significant effect (p > 0.05). In comparison, conditioned medium from iron-fed microglia (Iron-fed CM) caused a significant increase (p < 0.05) in the levels of α-syn protein detected. Conditioned medium from both C8B4 and primary microglia has a similar effect. Shown are the mean and S.E.M. of four separate experiments.