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. 2018 Aug 1;8(3):67. doi: 10.3390/biom8030067

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Cytokine Neutralization SH-SY5Y. Cells were treated with conditioned medium from iron-fed C8B4 microglia pre-treated with antibodies in an attempt to neutralize cytokines present that may be responsible for inducing increased expression of α-syn. We tested the effect of antibodies to TNFα, IL-1β and as a control, rabbit IgG. Medium was treated with 150 ng/mL of the specific antibody for one hour before applying to the SH-SY5Y cells. After 24 h western blot was used to assess the level of α-syn protein and tubulin to compare loading. On the antibodies tested only anti- TNFα caused a significant (p < 0.05) reduction in the level of α-syn detected. Shown are the mean and S.E.M. of four separate experiments.