Figure 10.

Inhibitors of signaling pathways. In order to assess the role of both the JNK pathway and NF-κB pathway in the increased expression of α-syn in response to conditioned medium from iron-fed C8B4 microglia, we applied a series of inhibitors of these pathways to SH-SY5Y cells during treatment with the conditioned medium. The compounds used were two inhibitors of the JNK pathways (JNK 2 and JNK 15) and one inhibitor of the NF-κB pathway (NF-κB 4). As the inhibitors were soluble in methanol we also included a methanol control at the highest volume used. After a 24 h treatment, the levels of α-syn and tubulin were assessed by western blot. The strongest inhibition was seen with the NF-κB 4 inhibitor. The inhibitory effect of JNK 2 was also significant (p < 0.05) but the effect of JNK 15 was not. Shown are the mean and S.E.M. of four separate experiments.