Table 1.
Cytokine released into conditioned medium.
| C8B4 Microglia | Primary Mouse Microglia | |||
|---|---|---|---|---|
| Cytokine (ng/mg) | Control | Iron-Fed | Control | Iron-Fed |
| TNFα | 142.82 ± 12.40 | 192.46 ± 5.52 * | 37.42 ± 3.74 | 84.19 ± 3.45 * |
| IFNγ | n.d. | n.d. | n.d. | n.d. |
| KC/GRO | n.d. | n.d. | 698 ± 16 | 2113 ± 398 * |
| IL-1b | 2.93 ± 0.27 | 3.83 ± 0.22 * | 0.99 ± 0.08 | 0.34 ± 0.03 * |
| IL-2 | n.d. | n.d. | n.d. | n.d. |
| IL-4 | n.d. | n.d. | n.d. | n.d. |
| IL-5 | n.d. | n.d. | n.d. | n.d. |
| IL-6 | 490.96 ± 49.77 | 430.77 ± 18.05 | 356.09 ±4 7.84 | 117.56 ± 24.22 * |
| IL-8 | n.d. | n.d. | n.d. | n.d. |
| IL-10 | 6.20 ± 1.04 | 0.84 ± 0.26 * | 5.00 ± 0.37 | 10.34 ± 0.98 * |
| IL-12p70 | n.d. | n.d. | n.d. | n.d. |
| IL-13 | n.d. | n.d. | n.d. | n.d. |
Serum free conditioned medium was generated from cultured microglia over 24 h and the levels of cytokines assessed with the Meso Scale Discovery (MSD) ELISA system. Cytokine concentrations were determined by comparison to a standard curve for each cytokine. Values were the concentration in the medium (ng/mL) divided by the concentration of the protein in the cells that were used to generate the conditioned medium (mg/mL). Shown are the mean (ng/mg) and S.E.M. for five (C8B4) and three (primary) different experiments. * Indicates a significant difference between control and iron-fed (p < 0.05). n.d. indicates not detectible due to the levels detected being below the level of lowest standard on the standard curve for that cytokine.