Figure 1.

Isolation of the cancer stem cell (CSC) inhibitor from aronia and assessment of the aronia extracts via mammosphere formation assay. (A) Assay of mammosphere formation inhibition using aronia extracts. The mammospheres were incubated with aronia extracts or DMSO. MDA-MB-231 cells were treated with aronia extracts or DMSO in CSC culture media for seven days. Images were obtained by microscopy at 10× magnification and were representative mammospheres (scale bar = 100 µm). (B) Isolation procedure of the mammosphere-forming inhibitor. (C) HPLC chromatogram of the inhibitor isolated from the aronia extracts.