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. 2018 Aug 26;19(9):2528. doi: 10.3390/ijms19092528

Figure 4.

Figure 4

Effect of 3-O-trans-p-coumaroyltormentic acid on the expression of CSC markers in breast cancer cell line. (A) The CD44high/CD24low cell population was analyzed by flow cytometry in MDA-MB-231 cells treated with either 3-O-trans-p-coumaroyltormentic acid (20 μM) or DMSO for two days. For FACS (Flow Cytometer) analysis, 50,000 cells were acquired. Gating was based on binding of the control antibody (red cross). (B) The effect of 3-O-trans-p-coumaroyltormentic acid on the ALDH-positive cell population. MDA-MB-231 cells were treated with either 3-O-trans-p-coumaroyltormentic acid (20 μM) or DMSO for two days and subjected to the ALDEFLUOR assay and FACS analysis. A set of representative flow cytometer dot plots is shown. The lower panel shows ALDH-positive cells in the presence of the ALDH inhibitor diethylaminobenzaldehyde (DEAB) as a negative control, and the upper panel represents ALDH-positive cells in the absence of DEAB. The ALDH-positive population was gated in the box (red dot line box).