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. 2018 Jul 6;6(3):59. doi: 10.3390/diseases6030059

Figure 1.

Figure 1

Trichostatin A (TSA) and valproic acid (VPA) pretreatment of peripheral blood mononuclear cells (PBMCs) infected with dengue virus (DENV-2) exhibit a decrease TNF-alpha and IL-6 production. PBMCs were treated with TSA (A) or VPA (B) for 3 h and then were infected with DENV-2. At 24 h post-infection, the supernatants were used to quantify cytokine production. Data is expressed as relative cytokine production compared with infected non-treated control cells. Error bars represent the mean ± SEM of three independent experiments. * p values were calculated using the non-parametric two-tailed Mann-Whitney test, comparing each experimental condition with the infected non-treated control. A p value < 0.05 was considered significant. PBMCs were treated with TSA (C) or VPA (D) for three hours and were then infected with DENV-2. At 3 h post-infection, total RNA was collected to quantify cytokine mRNA. Gene expression was normalized to human β-actin expression. The figure shown is representative data from one of three independent experiments.