Data Collection	QTRT1	QTRT1·Queuine
PDB-ID	6H42	6H45
X-ray source	Synchrotron (PETRA III, P14)	Synchrotron (PETRA III, P14)
Detector	Dectris Eiger	Dectris Eiger
Wavelength (Å)	0.97626	0.97626
Space group	C2	C2
Cell dimensions
a, b, c (Å)	127.70, 157.82, 47.77	127.50, 158.86, 47.62
α, β, γ (°)	90.00, 108.46, 90.00	90.00, 108.20, 90.00
Resolution (Å)	48.25–2.45 (2.55–2.45)	48.16–2.40 (2.50–2.40)
Rmeas (%)	10.6 (111.2)	5.8 (59.0)
I/σ(I)	10.3 (1.7)	17.3 (2.3)
CC1/2	99.6 (59.9)	99.9 (84.7)
Completeness (%)	99.4 (99.5)	93.1 (67.6)
Redundancy	4.5 (4.5)	3.3 (2.7)
Refinement
Resolution (Å)	48.25–2.45	48.16–2.40
No. of reflections	32849	32702
Rwork (%)	20.24	20.13
Rfree (%)	23.95	23.96
No. of atoms
Protein	6033	6010
Water	99	103
Ligand	-	40
Ions	15	13
B-factors (Å2)
Protein	67.3	58.0
Ligand	-	73.7
Water and other small molecules	61.7	71.7
r.m.s. deviations
Bond lengths (Å)	0.0037	0.0074
Bond angles (°)	0.643	0.814
MolProbity analysis
Ramachandran favored (%)	96.5	96.6
Ramachandran outliers (%)	0.0	0.0
Rotamer outliers (%)	1.37	1.99
Clash score	4.96	5.35