Table 2.
Media compositions used for initial isolations and subsequent growth assays and large-scale cultivation.
| Medium (Abbreviation) | Content Per L |
|---|---|
| Potato Dextrose Broth (PDB) | 26.5 g potato extract glucose (6.5 g potato extract, 20 g glucose) |
| Potato Dextrose Agar (PDA) | 26.5 g potato extract glucose, 20.0 g agar |
| ISP2 Broth | 4.0 g yeast extract, 10.0 g malt extract, 4.0 g glucose |
| ISP2 Agar | 4.0 g yeast extract, 10.0 g malt extract, 4.0 g glucose, 20.0 g agar |
| Chitin Agar | 4.0 g chitin, 0.7 g K2HPO4, 0.3 g KH2PO4, 0.57 g MgSO4·7H2O, 0.01 g FeSO4·7H2O, 0.0018 g ZnSO4·7H2O, 0.0016 g MnCl2·4H2O |
| Minimal Media | 2.0 g Na-acetate, 2.0 g NH4Cl 0.7 g K2HPO4, 0.3 g KH2PO4, 0.57 g MgSO4·7H2O, 0.01 g FeSO4·7H2O, 0.0018 g ZnSO4·7H2O, 0.0016 g MnCl2·4H2O |
| MC Agar | 5.0 g microcrystalline cellulose, 20.0 g agar |
| Soya Broth | 20.0 g soya flour, 20.0 g glucose, 5.0 g NaCl, 3.0 g CaCO3, 0.25 mL desmophen |