Induction of anemic rats by treatment with phenylhydrazine hydrochloride (PHZ) and the effects of ZnSO4 on their RBC levels. (A) Normal rats were injected with saline or a single PHZ (60 mg/kg body weight) solution at day 0; (B) After two days, the PHZ-induced anemic rats were further injected with saline or different concentrations of ZnSO4 solution. After another two days (at day 4), the rats were sacrificed, and RBC levels and other hematological indices were measured. *, significant difference between ZnSO4-injected and saline-injected anemic rats (p < 0.05, n = 6). This figure is representative of three different experiments.