Figure 4.

Expression of Mfsd2a in the brain and liver of Wt and 5xFAD mice after fish oil supplementation. The levels of Mfsd2a mRNA and protein were obtained by real-time polymerase chain reaction (RT-PCR) (A,C) and Western blotting (B,D), respectively in the brain (left panel) and liver (right panel) of Wt and 5xFAD animals. 03B2-actin served as an internal control of the mfsd2a gene expression and protein load. Data are presented as mean ± SEM (standard error of mean). * p < 0.05; n.s., not significant.