Figure 5.

Effects of the fish oil consumption on amyloid precursor protein APP, β-CTF and Aβ expression levels and plaques burden in the cortex of 5xFAD transgenic mice. (A) Representative Western blots and quantitative analysis of the full length APP and b-CTF fragment (Zymed, clone 695 that reacts with both human and mouse APP) and its proteolytic product Aβ (Invitrogen, clone H31L2). Quantification of APP band density was performed on less exposed X-ray films. (B) Representative low-magnification images of Thioflavin S stained plaques in the brain parietal cortex. Insets show plaque deposits in three different control (a–c) and FO supplemented (d–f) animals. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01. Magnification, ×5.