Table 2.

Sequences of the newly identified peptaibol compounds produced by T. koningiopsis SZMC 12500 and their similarities to known peptaibols available in the “Peptaibiotics Database”.

Peptide	M	[M+Na]+	[M+2Na]2+	b12	y7	rt-GK (min)	Area size	R1	R2	R3	R4	R5	R6	R7	R8	R9	R10	R11	R12	R13	R14	R15	R16	R17	R18	R19	Compound identical or positionally isomeric with	Reference
Koningiopsin Ia	1875.5	1898.5	960.75	1121.9	754.5	53.121	0.91%	AcAib	Ala	Ala	Aib	Vxx	Gln	Aib	Aib	Aib	Ser	Lxx	Aib	Pro	Vxx	Aib	Vxx	Gln	Gln	Lxxol	New: Trichorzianin TAP-14b: [Pheol]19→[Lxxol]19	[46]
Koningiopsin Ib	1889.5	1912.5	967.75	1121.5	768.5	54.267	5.96%	AcAib	Ala	Ala	Aib	Vxx	Gln	Aib	Aib	Aib	Ser	Lxx	Aib	Pro	Vxx	Aib	Lxx	Gln	Gln	Lxxol	New: Trichorzianin TA IVb: [Trpol]19→[Lxxol]19	[45]
																											New: Trichorzianin TA VII: [Pheol]19→[Lxxol]19	[45]
Koningiopsin IIa	1890.5	1913.5	968.25	1136.7	754.5	54.69	8.13%	AcAib	Ala	Ala	Aib	Aib	Gln	Aib	Aib	Lxx	Ser	Lxx	Aib	Pro	Vxx	Aib	Vxx	Gln	Gln	Lxxol	New: Trichorzianin TA IVb: [Aib]9→[Lxx]9 and [Trpol]19→[Lxxol]19	[45]
																											New: Trichostrigocin TSG-A, TSG-B:[Aib]3→[Ala]3 and [Aib]9→[Lxx]9	[41]
Koningiopsin IIb	1889.6	1912.6	967.8	1120.8	768.6	55.513	2.88%	AcAib	Ala	Ala	Aib	Aib	Gln	Aib	Aib	Aib	Vxx	Lxx	Aib	Pro	Vxx	Aib	Lxx	Gln	Gln	Lxxol	New: Trichorzianin TA IIIb, TA IIIc: [Ser]10→[Vxx]10 and	[45]
																											[Trpol]19→[Lxxol]19
																											New: Trichorzianin TA VIb: [Ser]10→[Vxx]10 and [Pheol]19→[Lxxol]19	[45]
																											New: Trichorzianin TAP-14a: [Ser]10→[Vxx]10 and [Pheol]19→[Lxxol]19	[46]
Koningiopsin IIIa	1891.6	1914.6	968.8	1122.7	755.6	55.593	0.97%	AcAib	Ala	Ala	Aib	Ala	Gln	Aib	Aib	Lxx	Ser	Lxx	Aib	Pro	Vxx	Aib	Vxx	Gln	Glu	Lxxol	New: Trichorzianin TAP-14b: [Aib]5→[Ala]5, [Aib]9→[Lxx]9,	[46]
																											[Gln]18→[Glu]18 and [Pheol]19→[Lxxol]19
																											New: Trichorzianin TB IIIc, TB IVb: [Aib]5→[Ala]5, [Aib]9→[Lxx]9, [Ile]16→[Vxx]16 and [Trpol]19→[Lxxol]19	[47]
																											New: Trichorzianin TB VIb, TB VII: [Aib]5→[Ala]5, [Aib]9→[Lxx]9, [Ile]16→[Vxx]16 and [Trpol]19→[Lxxol]19	[47]
																											New: Trichostrigocin TSG-A, TSG-B: [Aib]3→[Ala]3, [Ala]5→[Lxx]5, [Aib]9→[Lxx]9 and [Gln]18→[Glu]18	[41]
Koningiopsin IIIb	1873.6	1896.6	959.8	1120.8	754.5	55.936	2.03%	AcAib	Ala	Ala	Aib	Aib	Gln	Aib	Aib	Aib	Vxx	Lxx	Aib	Pro	Vxx	Aib	Vxx	Gln	Gln	Lxxol	New: Trichorzianin TAP-14b: [Ser]10→[Vxx]10 and [Pheol]19→[Lxxol]19	[46]
Koningiopsin IV	1903.6	1926.6	974.8	1136.7	768.6	56.317	12.27%	AcAib	Ala	Ala	Aib	Aib	Gln	Aib	Aib	Lxx	Ser	Lxx	Aib	Pro	Vxx	Aib	Lxx	Gln	Gln	Lxxol	New: Trichorzianin TA IIIb, TA IIIc: [Aib]9→[Lxx]9 and	[45]
																											[Trpol]19→[Lxxol]19 (Positional isomer of Koningiopsin Va)
																											New: Trichorzianin TA VIb: [Aib]9→[Lxx]9 and [Pheol]19→[Lxxol]19	[45]
																											New: Trichorzianin TAP-14a: [Aib]9→[Lxx]9 and [Pheol]19→[Lxxol]19	[46]
Koningiopsin Va	1903.6	1926.6	974.8	1136.8	768.6	56.739	47.94%	AcAib	Ala	Ala	Aib	Aib	Gln	Aib	Aib	Lxx	Ser	Lxx	Aib	Pro	Vxx	Aib	Lxx	Gln	Gln	Lxxol	New: (Positional isomer of Koningiopsin IV)	→Koningiopsin IV
Koningiopsin Vb	1905.6	1928.6	975.8	1136.8	769.6	57.463	5.95%	AcAib	Ala	Ala	Aib	Aib	Gln	Aib	Aib	Lxx	Ser	Lxx	Aib	Pro	Vxx	Aib	Lxx	Gln	Glu	Lxxol	New: Trichorzianin TB IIIc: [Aib]9→[Lxx]9, and [Trpol]19→[Lxxol]19	[47]
																											New: Trichorzianin TBVIb: [Aib]9→[Lxx]9, and [Pheol]19→[Lxxol]19	[47]
Koningiopsin VIa	1888.6	1911.6	967.3	1120.7	768.6	57.825	11.54%	AcAib	Ala	Ala	Aib	Aib	Gln	Aib	Aib	Lxx	Ala	Lxx	Aib	Pro	Vxx	Aib	Lxx	Gln	Gln	Lxxol	New: Trichorzianin TA IIIb, TA IIIc: [Aib]9→[Lxx]9, [Ser]10→[Ala]10	[45]
																											and [Trpol]19→[Lxxol]19
																											New: Trichorzianin TA VIb: [Aib]9→[Lxx]9, [Ser]10→[Ala]10 and [Pheol]19→[Lxxol]19	[45]
																											New: Trichorzianin TAP-14a: [Aib]9→[Lxx]9, [Ser]10→[Ala]10 and [Pheol]19→[Lxxol]19	[46]
Koningiopsin VIb	1888.7	1911.7	967.35	1120.9	769.5	58.448	1.41%	AcAib	Ala	Ala	Aib	Aib	Gln	Aib	Aib	Lxx	Ala	Lxx	Aib	Pro	Vxx	Aib	Lxx	Gln	Glu	Lxxol	New: Trichorzianin TB IIIc: [Aib]9→[Lxx]9, [Ser]10→[Ala]10 and	[47]
																											[Trpol]19→[Lxxol]19
																											New: Trichorzianin TB VIb: [Aib]9→[Lxx]9, [Ser]10→[Ala]10 and [Pheol]19→[Lxxol]19	[47]

Variable residues are underlined in the table header, minor sequence variants are underlined in the sequences. Amino acid exchanges in new sequences are italicised. Positions R14 and R15 were predicted based on the Comprehensive Peptaibotics Database [14].