Figure 1.

Interaction of CSFV p7 protein and host CAMLG detected by yeast two-hybrid system. (A) Yeast strain AH109 was transformed with either GAL4-binding domain (BD) fused to CSFV p7 (BD-p7), or mutated CSFV p7 construct as indicated or human lamin C (BD-LAM), a negative control and GAL4 activation domain (AD) fused to CAMLG (AD-CAMLG) or the negative control pGADT7 as indicated. 2 × 10⁶ yeast cells were spotted onto either SD-Ade/His/Leu/Trp plates (-ALTH), selective media for detecting protein-protein interactions or nonselective SD-Leu/Trp plates (-TL) (B) Schematic representation of CSFV p7 alanine mutants used in the present study. The name of each alanine p7 mutant is shown below the mutated amino acid residues for each particular mutant. All indicated residues were mutated to an alanine. The highlighted p7 mutants indicate alanine substitutions resulting in lack of binding of p7 to CAMLG in the yeast two-hybrid system.