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. 2018 Aug 16;6(3):65. doi: 10.3390/medsci6030065

Figure 4.

Figure 4

DFMO delays cell recovery in human OS cells. MG-63, U-2 OS and Saos-2 cells were exposed to 5 mM DFMO for six days and then reseeded in standard media. (A–C) Effect of treatment on cell recovery, 24, 48 and 72 h after removal of DFMO. Quantification of cell viability was determined by measuring relative light units (RLU) after the addition of RealTime-GloTM MT Viability assay reagent. Data represent three independent experiments done in triplicate (N = 9). Error bars represent standard deviation. MG-63 (A), U-2 OS (B) and Saos-2 (C) cell viability was markedly decreased after DFMO exposure; (D) to evaluate cell recovery beyond the 72 h time point, treated cells (six days with DFMO) or untreated control cells were washed, reseeded in standard media, and counted with a hemocytometer and trypan blue, seven days after DFMO removal. At this time point, previously treated cells still showed only minimal recovery compared to controls. Data represent three independent experiments done in triplicate (N = 9). Error bars represent standard error. * denotes statistically significant changes in cell proliferation compared with control (p < 0.05).