Figure 7.
Synergistic effect of Smad and SP-1 on the expression of α-SMA and Col-1 proteins in TGF-β1-stimulated NPDFs. The cells were seeded at 2 × 105 cells/mL and incubated with SIS3 (Smad 3 inhibitor), mithramycin (MTM) (SP-1 inhibitor), and SIS3 + MTM for 1 h prior to TGF-β1 stimulation (1 ng/mL). Following stimulation with TGF-β1 for 24 h, α-SMA protein and Col-1 protein expression was determined via western blotting. Untreated cells were used as a control (CON) and GAPDH was used as an internal control. Each value indicates the mean ± S.E.M. and is representative of results obtained from three independent experiments.