Figure 2.

Andro reduced the morphology damage, lactate dehydrogenase (LDH) release, MDA and NO levels in Aβ_1–42-treated PC12 cells. Cells were pre-treatment with or without 20 µM of Andro for 1 h before exposed to 10 μM of Aβ_1–42 for 24 h. Then, the cellular morphology was observed and photographed by inverted light microscopy with 10× magnification (A); Cells was detected by staining with Hoechst 33258 and visualized by fluorescence microscopy with 20 × magnification; (B). The release of LDH (C), the levels of MDA (D), and NO (E) were examined by using the reagent kits. * p < 0.05 versus the control; ^# p < 0.05, ^## p < 0.01 versus the Aβ_1–42 group was considered statistically significant differences.