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. 2018 Sep 19;19(9):2844. doi: 10.3390/ijms19092844

Figure 2.

Figure 2

Andro reduced the morphology damage, lactate dehydrogenase (LDH) release, MDA and NO levels in Aβ1–42-treated PC12 cells. Cells were pre-treatment with or without 20 µM of Andro for 1 h before exposed to 10 μM of Aβ1–42 for 24 h. Then, the cellular morphology was observed and photographed by inverted light microscopy with 10× magnification (A); Cells was detected by staining with Hoechst 33258 and visualized by fluorescence microscopy with 20 × magnification; (B). The release of LDH (C), the levels of MDA (D), and NO (E) were examined by using the reagent kits. * p < 0.05 versus the control; # p < 0.05, ## p < 0.01 versus the Aβ1–42 group was considered statistically significant differences.