Figure 3.
Andro attenuated Aβ1–42-induced reactive oxygen species (ROS) production and mitochondrial membrane potential (MMP) loss in PC12 cells. After pre-treatment with 20 μM of Andro for 1 h, PC12 cells were incubated with 10 μM Aβ1–42 for another 24 h. (A,B) Intracellular ROS level was determined by the fluorescent dye 2,7-dichlorofluorescein-diacetate (DCFH-DA) Reagent and visualized by fluorescence microscopy with 20× magnification, and Rosup was used as a positive control group; (C,D) MMP was determined by the JC-1 assay and visualized by fluorescence microscopy with 20× magnification. * p < 0.05, ** p < 0.01 versus the control; # p < 0.05 versus the Aβ1–42 group, were considered statistically significant differences.