Figure 4.

Andro alters Aβ_1–42-induced cytochrome C (Cytc), Bax, and p-Tau/Tau protein expression levels in PC12 cells. After pre-treatment with 20 μM of Andro for 1 h, PC12 cells were incubated with 10 μM of Aβ_1–42 for another 24 h. (A,C) The expression of Cytc, Bax, p-tau, and tau protein levels was determined by Western blotting with specific antibodies; (B,D) Quantification of Cytc, Bax, p-tau, and tau protein expression levels. * p < 0.05, ** p < 0.01 versus the control; ^# p< 0.05, ^## p < 0.01 versus the Aβ_1–42 group were considered statistically significant differences.