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. 2018 Sep 19;19(9):2844. doi: 10.3390/ijms19092844

Figure 7.

Figure 7

Effect of p62 small interfering RNA (siRNA) on Beclin 1 and LC3A/B-II protein expression levels in PC12 cells. (A,B) PC12 cells were transfected either with p62 siRNA (10 or 20 µM) or scrambled siRNA. Cells were then treated with Andro (20 µM) for 24 h, and p62, Beclin 1, and LC3A/B-II protein expression levels were evaluated by Western blot analysis; (C) After transfection with 20 µM of p62 siRNA, cells were then pre-treated with Andro (20 µM) for 1 h, followed by stimulation with Aβ (10 µM) for an additional 24 h. Then, Beclin 1 and LC3A/BII protein expression levels were evaluated by Western blot analysis. * p < 0.05 versus the blank control; # p < 0.05 versus the Andro + Aβ1–42 group were considered statistically significant differences.