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. 2018 Sep 19;19(9):2844. doi: 10.3390/ijms19092844

Figure 8.

Figure 8

Effect of Andro on Aβ1–42-induced p21 and Nrf2 mRNA and protein expression levels in PC12 cells. After pre-treatment with 20 μM of Andro for 1 h, PC12 cells were incubated with 10 μM of Aβ1–42 for another 24 h. Real-time qPCR analysis of the mRNA levels of p21 (A) and Nrf2 (B) in cells, and (C) Western blotting analysis of the protein levels of p62, Nrf2, and β-Actin in cells. Quantification of Nrf2 (D) and p21 (E) protein expression levels. * p < 0.05, ** p < 0.01 versus the control; # p < 0.05, ## p < 0.01 versus the Aβ1–42 group were considered statistically significant differences.