Figure 10.

Nrf2/p62 signaling pathway mediated by Andro involved autophagy induction in Aβ_1–42-stimulated PC12 cells. After transfection with 20 µM of Nrf2 siRNA (A) or p62 siRNA (B), cells were then pre-treated with Andro (20 µM) for 1 h followed by stimulation with Aβ (10 µM) for an additional 24 h, Beclin-1, LC3A/BII, Nrf2, and p21 protein expression levels were evaluated by Western blot analysis. ^# p < 0.05 versus the Andro + Aβ_1–42 group were considered statistically significant differences.