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. 2018 Jul 11;8(3):49. doi: 10.3390/biom8030049

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Lipid droplet (LD) content and dynamic change in Elongator mutants as compared to the wild type (WT). Elongator mutant strains were pre-grown to stationary phase (48 h) to attain a high LD content and then seeded at low density in YPD medium at 30 °C (A) Aliquots were withdrawn at stationary phase (48 h) or (B) after 5h of growth. (C) Lipid droplet dynamics of Elongator mutants was studied during the exponential phase (lipolytic phase) of growth. Stationary cells were seeded at 0.25 O.D 600 nm in YPD. Aliquots were withdrawn at the times indicated and fixed in formaldehyde 3.7%. Afterwards the LD index was measured with the liquid fluorescent recovery (LFR) assay using Bodipy 493/503 as a probe [2] and normalized to WT. The mean ± (standard deviation) S.D. of three independent experiments is shown (* p < 0.05).