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. 2018 Sep 12;19(9):2722. doi: 10.3390/ijms19092722

Figure 4.

Figure 4

Confocal laser scanning microscopy: representative images of rat gut sections after 0.5 and 1 h incubation in Ussing chamber. Negative controls obtained omitting primary antibodies are shown in double line squares. Individual fluorescent channels: green (SRM), red (GP2 + cells) and blue (nuclei). (A) bidimensional image of the maximum intensity projection. Scale bar 25 µm. (B) Images acquired in one Z-plane (38th of 59, 22nd of 64, incubated 0.5 h and 1 h, respectively) at a higher magnification. Scale bar 10 µm.