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AWRK6 inhibited LPS-induced apoptosis in mice liver. (A) AWRK6 (10 mg/kg) treatment for 24 h reduced DNA fragmentation induced by LPS (50 mg/kg), assayed by TUNEL assay. (B) The results of TUNEL assay were analyzed by ImageJ. (C) The protein levels of cleaved-caspase 9, BAX and Bcl-2 were analyzed by western blotting. (D) The quantification of western blotting results was carried out using ImageJ. * p < 0.05 compared with the LPS groups. Scale bar indicates 100 μm.
