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. 2018 Sep 7;19(9):2661. doi: 10.3390/ijms19092661

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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AWRK6 inhibited LPS-induced liver cell apoptosis in HepG2 cells. (A) The viabilities of HepG2 liver cells treated with LPS (40 μg/mL) with/without AWRK6 for 24 h, examined by MTT assay. (B) The cells treated with LPS and AWRK6 (200 μg/mL) were observed under phase contrast microscope. (C) The cell apoptosis was detected by Annexin V-FITC/PI staining followed by fluorescence microscopy. (D) The apoptotic cell number in the results of Annexin V-FITC/PI staining was analyzed by ImageJ. (E) The protein levels of cleaved-caspase 9, BAX and Bcl-2 were analyzed by western blotting. (F) The results of western blotting were quantified using ImageJ. Bar indicates 100 μm. * p < 0.05 compared with the LPS groups.

AWRK6 inhibited LPS-induced liver cell apoptosis in HepG2 cells. (A) The viabilities of HepG2 liver cells treated with LPS (40 μg/mL) with/without AWRK6 for 24 h, examined by MTT assay. (B) The cells treated with LPS and AWRK6 (200 μg/mL) were observed under phase contrast microscope. (C) The cell apoptosis was detected by Annexin V-FITC/PI staining followed by fluorescence microscopy. (D) The apoptotic cell number in the results of Annexin V-FITC/PI staining was analyzed by ImageJ. (E) The protein levels of cleaved-caspase 9, BAX and Bcl-2 were analyzed by western blotting. (F) The results of western blotting were quantified using ImageJ. Bar indicates 100 μm. * p < 0.05 compared with the LPS groups.