Fig. 7.

Northern blot analysis of hGR mRNA. 10 μg of poly(A)⁺ mRNA from human HT1080 fibroblast cells, collected after 24 h without ( − ) or with ( + ) treatment with 10 μM dexamethasone, was electrophoresed through a 0.8% agarose/1% formaldehyde gel, stained with acridine orange (120 μg ml⁻¹) and transferred to nitrocellulose. The filter was hybridized overnight with nick-translated hGR1.2 (10⁶ c.p.m. ml⁻¹, specific activity 10⁸ c.p.m. μg⁻¹) and washed with 2 × SSC at 68 °C. Sizes were estimated from human fibronectin mRNA (7.8 kb), and 28 S (5.0 kb) and 18 S (2.1 kb) ribosomal RNAs.