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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: Small. 2018 Jul 15;14(32):e1800703. doi: 10.1002/smll.201800703

Figure 5. AcDex-DXM release drug for eight hours and reduces cytokine production as a long-term prophylaxis.

Figure 5.

Drug was released by pH 6 after an 8-day pre-incubation as measured by LC-MS over 8 hours (A). In BMDMs (B) or C166 endothelial cells (C), treatments (equivalent doses of 40 nM DXM) were added six (B) or eight (C) days before first stimulations. Stimulation consisted of LPS (0.1 μg/ml) for 2–3 hours, and then MSU crystals were added (0.2 mg/ml) overnight, and supernatants were saved at −80°C and then analyzed for IL-1β (B) or CXCL1 (C) levels by ELISA. Data is presented as pg/ml, (***p < 0.001, *p < 0.05).