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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Free Radic Biol Med. 2018 Aug 7;126:187–201. doi: 10.1016/j.freeradbiomed.2018.08.001

Figure 3.

Figure 3.

Effects of mechanical stretch on the levels of intracellular antioxidant enzymes in BM-MSCs. Cells were subjected to cyclic stretch for 2 h per day at the magnitudes of 2.5%, 5%, and 10%. Cells cultured under static conditions served as the control (CTRL). (A-B) After 3 days of stretch, the mRNA levels of SOD1 (A) and SOD2 (B) in BM-MSCs were quantified using RT-qPCR. (C) Effects of cyclic stretch on the activity of SOD in BM-MSCs. (D) The protein levels of SOD1 and SOD2 in stretch-treated BM-MSCs were determined by western blot. (E-F) The mRNA levels of CATand GPX1 (F) in BM-MSCs were quantified using RT-qPCR. (G) The protein levels of CAT and GPx1 in stretch-treated BM-MSCs were determined by western blot. Data are presented as the mean ± S.E.M. of four independent experiments (n = 4) in RT-qPCR and SOD activity experiments. Statistically significant differences are indicated by * where p < 0.05 between the indicated groups and # where p < 0.05 vs. the CTRL group.