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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Free Radic Biol Med. 2018 Aug 7;126:187–201. doi: 10.1016/j.freeradbiomed.2018.08.001

Figure 4.

Figure 4.

Stretch-induced antioxidant effect on BM-MSCs was through activation of the AMPK-SIRT1 signaling pathway. Cells were exposed to cyclic stretch for 3 days (2 h per day) at the magnitudes of 2.5%, 5%, and 10%. Cells cultured under static conditions served as the control (CTRL). (A) Intracellular ROS of stretch-treated BM-MSCs were determined by flow cytometry. (B) Quantification data showed that cyclic stretch of 2.5% and 5% attenuated the levels of intracellular ROS in BM-MSCs. Data are presented as the mean ± S.E.M. of four independent experiments (n = 4) in ROS assays. (C) The mRNA levels of SIRT1 in BM-MSCs were quantified using RT-qPCR. Data are presented as the mean ± S.E.M. of four independent experiments (n = 4) in RT-qPCR experiments. (D-E) The protein levels of SIRT1 in BM-MSCs were determined using western blot assays. (F-G) The phosphorylation levels of AMPK in BM-MSCs were determined using western blot assays. Data are presented as the mean ± S.E.M. of three independent experiments (n = 3) in western blot assays.Statistically significant differences are indicated by * where p < 0.05 between the indicated groups and # where p < 0.05 vs. the CTRL group.