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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Free Radic Biol Med. 2018 Aug 7;126:187–201. doi: 10.1016/j.freeradbiomed.2018.08.001

Figure 6.

Figure 6.

Effects of pre-treatment with Compound C (CC) or Sirtinol on stretch-activated AMPK-SIRT1 signaling pathway. BM-MSCs were pre-treated with 10 μM CC (an AMPK inhibitor) or 40 μM Sirtinol (a SIRT1 inhibitor) for 2 h, and then subjected to cyclic stretch of 5% for 3 days (2 h per day). (A) The phosphorylation levels of AMPK were measured by western blot assays. (B-C) Quantification of phosphorylation levels and total expression of AMPK in BM-MSCs. (D) The mRNA levels of SIRT1 were quantified using RT-qPCR. Data are presented as the mean ± S.E.M. of four independent experiments (n = 4) in RT-qPCR experiments. (E-F) The protein levels of SIRT1 were quantified by western blot assays. Data are presented as the mean ± S.E.M. of three independent experiments (n = 3) in western blot assays. Statistically significant differences are indicated by * where p < 0.05 between the indicated groups and # where p < 0.05 vs. the untreated cells.