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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Free Radic Biol Med. 2018 Aug 7;126:187–201. doi: 10.1016/j.freeradbiomed.2018.08.001

Figure 7.

Figure 7.

Inhibition of AMPK or SIRT1 abrogated stretch-induced antioxidant responses in BM-MSCs. Cells were pre-treated with CC or Sirtinol for 2 h, and then subjected to cyclic stretch of 5% for 3 days (2 h per day). (A-D) The mRNA levels of SOD1 (A), SOD2 (B), CAT (C), and GPX1 (D) in BM-MSCs were quantified using RT-qPCR. Data are presented as the mean ± S.E.M. of four independent experiments (n = 4) in RT-qPCR experiments. (E-F) The protein levels of SOD1, SOD2, CAT, and GPx1 in BM-MSCs were determined using western blot assays. Statistically significant differences are indicated by * where p < 0.05 between the indicated groups and # where p < 0.05 vs. the untreated cells. Data are presented as the mean ± S.E.M. of three independent experiments (n = 3) in western blot assays. Statistically significant differences are indicated by * where p < 0.05 between the indicated groups and # where p < 0.05 vs. the untreated cells.