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. Author manuscript; available in PMC: 2018 Sep 30.
Published in final edited form as: Genes Immun. 2018 Mar 30;20(3):181–197. doi: 10.1038/s41435-018-0023-2

FIGURE 3.

FIGURE 3

IL12RB1 expression in the Jurkat T cell line is allele-biased and results in the production of Isoform 1 and Isoform 2. (A) Jurkat cell IL12RB1 gDNA and mRNA were sequenced and analyzed in manner identical to that described in FIG 1A–C. The results demonstrate that Jurkat cells are polymorphic at 9 gDNA positions (18170384, 18170755, 18180413, 18180451, 18186575, 18186618, 18188408, 18194255 and 18197635); like primary human tissue, the range of ARFgDNA values for these 9 positions was consistent with equal allelic representation (44%-56%), while the ARFmRNA values were biased towards one allele (95%-100%) at the expense of the other allele (0-5%). (B) Jurkat mRNA was converted to cDNA, which was then used for Isoform 1 and Isoform 2 PCR amplification. Shown are the electrophoresis patterns of (lane 1) a ladder control and (lane 2) Jurkat T cell cDNA amplicons; (lane 3) a “no RT control” was also amplified to confirm cDNA specificity.